Diego and Alicia find out about tree frogs once Diego learns that there are tree frogs in trouble. Alicia figures out that they are Red-Eyed Tree Frog's, and Diego figures out that the wind blew the tree frogs off of their tree and they are going down the river. Alicia tries to find the tree fogs a new home while Diego and Baby Jaguar try to save them. Along their way, they face several new challenges, such as finding a way to get across a muddy pit, jumping over rolling coconuts, finding their way into a pyramid that the tree frogs went into, and finding their way through the pyramid to get to the tree frogs.

Diego and a sloth, Sammy, try to save a mother sloth and her baby before a mudslide hits their tree. On the way they face several challenges. They must row across a rocky lake, tiptoe quietly around birds, and find the right path to go down. They also have to find an animal that can help them climb a rocky mountain.

When Diego learns of a little chinchilla who got lost from her family, he helps the chinchilla to find her way home to Chinchilla Mountain to be reunited with her family. Along their way, they face new challenges! Some of those include choosing the right path with the wrong path being filled with snakes, and finding a way up Chinchilla Mountain to get the little chinchilla back with her family.

When Diego learns that a baby humpback whale is in trouble, he takes off right away to save it. Along his way, he faces exciting new challenges. Some of those challenges include finding the right path to get to the whale, finding an animal to help him get around some jellyfish, and having to out swim sharks! The trouble doesn't stop there though, as Diego has to also figure out a way to get the baby humpback whale off of the beach and back into the ocean.

Baby Jaguar is full of pride when he thinks that he is big enough to get to the top of Jaguar Mountain. Diego and Baby Jaguar's mother agree to meet him at the top of Jaguar Mountain. Does Baby Jaguar have the skills to make it up to the top, or will the obstacles prove to be too much trouble for him to handle?